Functional Annotation with biomartr

2022-02-22

Functional Annotation Retrieval from Ensembl Biomart

NOTE: To make sure that you have a sufficiently stable (internet) connection between R and the respective databases, please set the default timeout setting on your local machine from 60sec to at least 30000sec before running any retrieval functions via:

options(timeout = 30000)

Getting Started

The Ensembl Biomart database enables users to retrieve a vast diversity of annotation data for specific organisms. Initially, Steffen Durinck and Wolfgang Huber provided a powerful interface between the R language and Ensembl Biomart by implementing the R package biomaRt.

The purpose of the biomaRt package was to mimic the ENSEMBL BioMart database structure to construct queries that can be sent to the Application Programming Interface (API) of BioMart. Although, this procedure was very useful in the past, it seems not intuitive from an organism centric point of view. Usually, users wish to download functional annotation for a particular organism of interest. However, the BioMart and thus the biomaRt package require that users already know in which mart and dataset the organism of interest will be found which requires significant efforts of searching and screening. In addition, once the mart and dataset of a particular organism of interest were found and specified the user must again learn which attribute has to be specified to retrieve the functional annotation information of interest.

The new functionality implemented in the biomartr package aims to overcome this search bottleneck by extending the functionality of the biomaRt package. The new biomartr package introduces a more organism cantered annotation retrieval concept which does not require to screen for marts, datasets, and attributes beforehand. With biomartr users only need to specify the scientific name of the organism of interest to then retrieve available marts, datasets, and attributes for the corresponding organism of interest.

This paradigm shift enables users to quickly construct queries to the BioMart database without having to learn the particular database structure and organization of BioMart.

The following sections will introduce users to the functionality and data retrieval precedures of biomartr and will show how biomartr extends the functionality of the initial biomaRt package.

The old biomaRt query methodology

The best way to get started with the old methodology presented by the established biomaRt package is to understand the workflow of its data retrieval process. The query logic of the biomaRt package derives from the database organization of Ensembl Biomart which stores a vast diversity of annotation data for specific organisms. In detail, the Ensembl Biomart database is organized into so called:
marts, datasets, and attributes. Marts denote a higher level category of functional annotation such as SNP (e.g. for functional annotation of particular single nucleotide polymorphisms (SNPs)) or FUNCGEN (e.g. for functional annotation of regulatory regions or relationsships of genes). Datasets denote the particular species of interest for which functional annotation is available within this specific mart. It can happen that datasets (= particular species of interest) are available in one mart (= higher category of functional annotation) but not in an other mart. For the actual retrieval of functional annotation information users must then specify the type of functional annotation information they wish to retrieve. These types are called attributes in the biomaRt notation.

Hence, when users wish to retrieve information for a specific organism of interest, they first need to specify a particular mart and dataset in which the information of the corresponding organism of interest can be found. Subsequently they can specify the attributes argument to retrieve a particular type of functional annotation (e.g. Gene Ontology terms).

The following section shall illustrate how marts, datasets, and attributes could be explored using biomaRt before the biomartr package existed.

The availability of marts, datasets, and attributes can be checked by the following functions:

# install the biomaRt package       
# source("https://bioconductor.org/biocLite.R")     
# biocLite("biomaRt")       
# load biomaRt      
library(biomaRt)        
# look at top 10 databases      
head(biomaRt::listMarts(host = "https://www.ensembl.org"), 10)      

Users will observe that several marts providing annotation for specific classes of organisms or groups of organisms are available.

For our example, we will choose the hsapiens_gene_ensembl mart and list all available datasets that are element of this mart.

head(biomaRt::listDatasets(biomaRt::useMart("ENSEMBL_MART_ENSEMBL", host = "https://www.ensembl.org")), 10)     

The useMart() function is a wrapper function provided by biomaRt to connect a selected BioMart database (mart) with a corresponding dataset stored within this mart.
We select dataset hsapiens_gene_ensembl and now check for available attributes (annotation data) that can be accessed for Homo sapiens genes.

head(biomaRt::listAttributes(biomaRt::useDataset(
                                         dataset = "hsapiens_gene_ensembl",         
                                         mart    = useMart("ENSEMBL_MART_ENSEMBL",      
                                         host    = "https://www.ensembl.org"))), 10)        

Please note the nested structure of this attribute query. For an attribute query procedure an additional wrapper function named useDataset() is needed in which useMart() and a corresponding dataset needs to be specified. The result is a table storing the name of available attributes for
Homo sapiens as well as a short description.

Furthermore, users can retrieve all filters for Homo sapiens that can be specified by the actual BioMart query process.

 head(biomaRt::listFilters(biomaRt::useDataset(dataset = "hsapiens_gene_ensembl",       
                                               mart    = useMart("ENSEMBL_MART_ENSEMBL",        
                                               host    = "https://www.ensembl.org"))), 10)      

After accumulating all this information, it is now possible to perform an actual BioMart query by using the getBM() function.

In this example we will retrieve attributes: start_position,end_position and description
for the Homo sapiens gene "GUCA2A".

Since the input genes are ensembl gene ids, we need to specify the filters argument filters = "hgnc_symbol".

 # 1) select a mart and data set        
 mart <- biomaRt::useDataset(dataset = "hsapiens_gene_ensembl",         
                    mart    = useMart("ENSEMBL_MART_ENSEMBL",       
                    host    = "https://www.ensembl.org"))       
        
 # 2) run a biomart query using the getBM() function        
 # and specify the attributes and filter arguments      
 geneSet <- "GUCA2A"        
        
 resultTable <- biomaRt::getBM(attributes = c("start_position","end_position","description"),       
                      filters    = "hgnc_symbol",       
                      values     = geneSet,         
                      mart       = mart)        
        
 resultTable        

When using getBM() users can pass all attributes retrieved by listAttributes() to the attributes argument of the getBM() function.

Extending biomaRt using the new query system of the biomartr package

Getting Started with biomartr

This query methodology provided by Ensembl Biomart and the biomaRt package is a very well defined approach for accurate annotation retrieval. Nevertheless, when learning this query methodology it (subjectively) seems non-intuitive from the user perspective. Therefore, the biomartr package provides another query methodology that aims to be more organism centric.

Taken together, the following workflow allows users to perform fast BioMart queries for attributes using the biomart() function implemented in this biomartr package:

  1. get attributes, datasets, and marts via : organismAttributes()

  2. choose available biological features (filters) via: organismFilters()

  3. specify a set of query genes: e.g. retrieved with getGenome(), getProteome() or getCDS()

  4. specify all arguments of the biomart() function using steps 1) - 3) and perform a BioMart query

Note that dataset names change very frequently due to the update of dataset versions. So in case some query functions do not work properly, users should check with organismAttributes(update = TRUE) whether or not their dataset name has been changed. For example, organismAttributes("Homo sapiens", topic = "id", update = TRUE) might reveal that the dataset ENSEMBL_MART_ENSEMBL has changed.

Retrieve marts, datasets, attributes, and filters with biomartr

Retrieve Available Marts

The getMarts() function allows users to list all available databases that can be accessed through BioMart interfaces.

# load the biomartr package
library(biomartr)

# list all available databases
biomartr::getMarts()
     mart                  version                       
   <chr>                 <chr>                         
 1 ENSEMBL_MART_ENSEMBL  Ensembl Genes 104             
 2 ENSEMBL_MART_MOUSE    Mouse strains 104             
 3 ENSEMBL_MART_SEQUENCE Sequence                      
 4 ENSEMBL_MART_ONTOLOGY Ontology                      
 5 ENSEMBL_MART_GENOMIC  Genomic features 104          
 6 ENSEMBL_MART_SNP      Ensembl Variation 104         
 7 ENSEMBL_MART_FUNCGEN  Ensembl Regulation 104        
 8 plants_mart           Ensembl Plants Genes 51       
 9 plants_variations     Ensembl Plants Variations 51  
10 fungi_mart            Ensembl Fungi Genes 51        
11 fungi_variations      Ensembl Fungi Variations 51   
12 protists_mart         Ensembl Protists Genes 51     
13 protists_variations   Ensembl Protists Variations 51
14 metazoa_mart          Ensembl Metazoa Genes 51      
15 metazoa_variations    Ensembl Metazoa Variations 51 

Retrieve Available Datasets from a Specific Mart

Now users can select a specific database to list all available data sets that can be accessed through this database. In this example we choose the ENSEMBL_MART_ENSEMBL database.

head(biomartr::getDatasets(mart = "ENSEMBL_MART_ENSEMBL") , 5)
 dataset                 description                        version     
  <chr>                   <chr>                              <chr>       
1 fcatus_gene_ensembl     Cat genes (Felis_catus_9.0)        Felis_catus…
2 umaritimus_gene_ensembl Polar bear genes (UrsMar_1.0)      UrsMar_1.0  
3 ogarnettii_gene_ensembl Bushbaby genes (OtoGar3)           OtoGar3     
4 lcrocea_gene_ensembl    Large yellow croaker genes (L_cro… L_crocea_2.0
5 sformosus_gene_ensembl  Asian bonytongue genes (fSclFor1.… fSclFor1.1 

Now you can select the dataset hsapiens_gene_ensembl and list all available attributes that can be retrieved from this dataset.

tail(biomartr::getDatasets(mart = "ENSEMBL_MART_ENSEMBL") , 38)
1 csabaeus_gene_ensembl    Vervet-AGM genes (ChlSab1.1)  ChlSab1.1      
 2 chircus_gene_ensembl     Goat genes (ARS1)             ARS1           
 3 mmulatta_gene_ensembl    Macaque genes (Mmul_10)       Mmul_10        
 4 mmonoceros_gene_ensembl  Narwhal genes (NGI_Narwhal_1) NGI_Narwhal_1  
 5 csemilaevis_gene_ensembl Tongue sole genes (Cse_v1.0)  Cse_v1.0       
 6 cpbellii_gene_ensembl    Painted turtle genes (Chryse… Chrysemys_pict…
 7 clanigera_gene_ensembl   Long-tailed chinchilla genes… ChiLan1.0      
 8 catys_gene_ensembl       Sooty mangabey genes (Caty_1… Caty_1.0       
 9 tguttata_gene_ensembl    Zebra finch genes (bTaeGut1_… bTaeGut1_v1.p  
10 nleucogenys_gene_ensembl Gibbon genes (Nleu_3.0)       Nleu_3.0       
# … with 28 more rows

Retrieve Available Attributes from a Specific Dataset

Now that you have selected a database (hsapiens_gene_ensembl) and a dataset (hsapiens_gene_ensembl), users can list all available attributes for this dataset using the getAttributes() function.

# show all elements of the data.frame
options(tibble.print_max = Inf)
# list all available attributes for dataset: hsapiens_gene_ensembl
head( biomartr::getAttributes(mart    = "ENSEMBL_MART_ENSEMBL", 
                              dataset = "hsapiens_gene_ensembl"), 10 )
Starting retrieval of attribute information from mart ENSEMBL_MART_ENSEMBL and dataset hsapiens_gene_ensembl ...
                            name                  description
1                ensembl_gene_id               Gene stable ID
2        ensembl_gene_id_version       Gene stable ID version
3          ensembl_transcript_id         Transcript stable ID
4  ensembl_transcript_id_version Transcript stable ID version
5             ensembl_peptide_id            Protein stable ID
6     ensembl_peptide_id_version    Protein stable ID version
7                ensembl_exon_id               Exon stable ID
8                    description             Gene description
9                chromosome_name     Chromosome/scaffold name
10                start_position              Gene start (bp)

Retrieve Available Filters from a Specific Dataset

Finally, the getFilters() function allows users to list available filters for a specific dataset that can be used for a biomart() query.

# show all elements of the data.frame
options(tibble.print_max = Inf)
# list all available filters for dataset: hsapiens_gene_ensembl
head( biomartr::getFilters(mart    = "ENSEMBL_MART_ENSEMBL", 
                           dataset = "hsapiens_gene_ensembl"), 10 )
Starting retrieval of filters information from mart ENSEMBL_MART_ENSEMBL and dataset hsapiens_gene_ensembl ...
                 name                            description
1     chromosome_name               Chromosome/scaffold name
2               start                                  Start
3                 end                                    End
4          band_start                             Band Start
5            band_end                               Band End
6        marker_start                           Marker Start
7          marker_end                             Marker End
8       encode_region                          Encode region
9              strand                                 Strand
10 chromosomal_region e.g. 1:100:10000:-1, 1:100000:200000:1

Organism Specific Retrieval of Information

In most use cases, users will work with a single or a set of model organisms. In this process they will mostly be interested in specific annotations for this particular model organism. The organismBM() function addresses this issue and provides users with an organism centric query to marts and datasets which are available for a particular organism of interest.

Note that when running the following functions for the first time, the data retrieval procedure will take some time, due to the remote access to BioMart. The corresponding result is then saved in a *.txt file named _biomart/listDatasets.txt within the tempdir() folder, allowing subsequent queries to be performed much faster. The tempdir() folder, however, will be deleted after a new R session was established. In this case the inital call of the subsequent functions again will take time to retrieve all organism specific data from the BioMart database.

This concept of locally storing all organism specific database linking information available in BioMart into an internal file allows users to significantly speed up subsequent retrieval queries for that particular organism.

# show all elements of the data.frame
options(tibble.print_max = Inf)
# retrieving all available datasets and biomart connections for
# a specific query organism (scientific name)
biomartr::organismBM(organism = "Homo sapiens")
 Starting retrieval of all available BioMart datasets for Homo sapiens ...
Datasets for the following marts will be retrieved:                                                         
                    mart                        version
1   ENSEMBL_MART_ENSEMBL              Ensembl Genes 104
2     ENSEMBL_MART_MOUSE              Mouse strains 104
3  ENSEMBL_MART_SEQUENCE                       Sequence
4  ENSEMBL_MART_ONTOLOGY                       Ontology
5   ENSEMBL_MART_GENOMIC           Genomic features 104
6       ENSEMBL_MART_SNP          Ensembl Variation 104
7   ENSEMBL_MART_FUNCGEN         Ensembl Regulation 104
8            plants_mart        Ensembl Plants Genes 51
9      plants_variations   Ensembl Plants Variations 51
10            fungi_mart         Ensembl Fungi Genes 51
11      fungi_variations    Ensembl Fungi Variations 51
12         protists_mart      Ensembl Protists Genes 51
13   protists_variations Ensembl Protists Variations 51
14          metazoa_mart       Ensembl Metazoa Genes 51
Processing mart ENSEMBL_MART_ENSEMBL ...
Processing mart ENSEMBL_MART_MOUSE ...
Processing mart ENSEMBL_MART_SEQUENCE ...
Processing mart ENSEMBL_MART_ONTOLOGY ...
Processing mart ENSEMBL_MART_GENOMIC ...
Processing mart ENSEMBL_MART_SNP ...
Processing mart ENSEMBL_MART_FUNCGEN ...
Processing mart plants_mart ...
Processing mart plants_variations ...
Processing mart fungi_mart ...
Processing mart fungi_variations ...
Processing mart protists_mart ...
Processing mart protists_variations ...
Processing mart metazoa_mart ...
# A tibble: 15 × 5                                                                                          
   organism_name description               mart      dataset      version
   <chr>         <chr>                     <chr>     <chr>        <chr>  
 1 hsapiens      Human genes (GRCh38.p13)  ENSEMBL_… hsapiens_ge… GRCh38…
 2 hsapiens      Human sequences (GRCh38.… ENSEMBL_… hsapiens_ge… GRCh38…
 3 hsapiens      encode                    ENSEMBL_… hsapiens_en… GRCh38…
 4 hsapiens      marker_feature_end        ENSEMBL_… hsapiens_ma… GRCh38…
 5 hsapiens      marker_feature            ENSEMBL_… hsapiens_ma… GRCh38…
 6 hsapiens      karyotype_end             ENSEMBL_… hsapiens_ka… GRCh38…
 7 hsapiens      karyotype_start           ENSEMBL_… hsapiens_ka… GRCh38…
 8 hsapiens      Human Somatic Short Vari… ENSEMBL_… hsapiens_sn… GRCh38…
 9 hsapiens      Human Structural Variant… ENSEMBL_… hsapiens_st… GRCh38…
10 hsapiens      Human Short Variants (SN… ENSEMBL_… hsapiens_snp GRCh38…
11 hsapiens      Human Somatic Structural… ENSEMBL_… hsapiens_st… GRCh38…
12 hsapiens      Human Regulatory Evidenc… ENSEMBL_… hsapiens_pe… GRCh38…
13 hsapiens      Human Regulatory Feature… ENSEMBL_… hsapiens_re… GRCh38…
14 hsapiens      Human Other Regulatory R… ENSEMBL_… hsapiens_ex… GRCh38…
15 hsapiens      Human miRNA Target Regio… ENSEMBL_… hsapiens_mi… GRCh38…

The result is a table storing all marts and datasets from which annotations can be retrieved for Homo sapiens. Furthermore, a short description as well as the version of the data set being accessed (very useful for publications) is returned.

Users will observe that 3 different marts provide 6 different datasets storing annotation information for Homo sapiens.

**_Please note__*, however, that scientific names of organisms must be written correctly! For ex. “Homo Sapiens” will be treated differently (not recognized) than “Homo sapiens” (recognized).__

Similar to the biomaRt package query methodology, users need to specify attributes and filters to be able to perform accurate BioMart queries. Here the functions organismAttributes() and organismFilters() provide useful and intuitive concepts to obtain this information.

# show all elements of the data.frame
options(tibble.print_max = Inf)
# return available attributes for "Homo sapiens"
head(biomartr::organismAttributes("Homo sapiens"), 20)
1 ensembl_gene_id               Gene stable ID         hsapiens_ge… ENSEMBL_M…
 2 ensembl_gene_id_version       Gene stable ID version hsapiens_ge… ENSEMBL_M…
 3 ensembl_transcript_id         Transcript stable ID   hsapiens_ge… ENSEMBL_M…
 4 ensembl_transcript_id_version Transcript stable ID … hsapiens_ge… ENSEMBL_M…
 5 ensembl_peptide_id            Protein stable ID      hsapiens_ge… ENSEMBL_M…
 6 ensembl_peptide_id_version    Protein stable ID ver… hsapiens_ge… ENSEMBL_M…
 7 ensembl_exon_id               Exon stable ID         hsapiens_ge… ENSEMBL_M…
 8 description                   Gene description       hsapiens_ge… ENSEMBL_M…
 9 chromosome_name               Chromosome/scaffold n… hsapiens_ge… ENSEMBL_M…
10 start_position                Gene start (bp)        hsapiens_ge… ENSEMBL_M…
11 end_position                  Gene end (bp)          hsapiens_ge… ENSEMBL_M…
12 strand                        Strand                 hsapiens_ge… ENSEMBL_M…
13 band                          Karyotype band         hsapiens_ge… ENSEMBL_M…
14 transcript_start              Transcript start (bp)  hsapiens_ge… ENSEMBL_M…
15 transcript_end                Transcript end (bp)    hsapiens_ge… ENSEMBL_M…
16 transcription_start_site      Transcription start s… hsapiens_ge… ENSEMBL_M…
17 transcript_length             Transcript length (in… hsapiens_ge… ENSEMBL_M…
18 transcript_tsl                Transcript support le… hsapiens_ge… ENSEMBL_M…
19 transcript_gencode_basic      GENCODE basic annotat… hsapiens_ge… ENSEMBL_M…
20 transcript_appris             APPRIS annotation      hsapiens_ge… ENSEMBL_M…

Users will observe that the organismAttributes() function returns a data.frame storing attribute names, data sets, and marts which are available for Homo sapiens. After the ENSEMBL release 87 the ENSEMBL_MART_SEQUENCE service provided by Ensembl does not work properly and thus the organismAttributes() function prints out warning messages to make the user aware when certain marts provided by Ensembl do not work properly, yet.

An additional feature provided by organismAttributes() is the topic argument. The topic argument allows users to to search for specific attributes, topics, or categories for faster filtering.

# show all elements of the data.frame
options(tibble.print_max = Inf)
# search for attribute topic "id"
head(biomartr::organismAttributes("Homo sapiens", topic = "id"), 20)
  name                          description            dataset      mart      
   <chr>                         <chr>                  <chr>        <chr>     
 1 ensembl_gene_id               Gene stable ID         hsapiens_ge… ENSEMBL_M…
 2 ensembl_gene_id_version       Gene stable ID version hsapiens_ge… ENSEMBL_M…
 3 ensembl_transcript_id         Transcript stable ID   hsapiens_ge… ENSEMBL_M…
 4 ensembl_transcript_id_version Transcript stable ID … hsapiens_ge… ENSEMBL_M…
 5 ensembl_peptide_id            Protein stable ID      hsapiens_ge… ENSEMBL_M…
 6 ensembl_peptide_id_version    Protein stable ID ver… hsapiens_ge… ENSEMBL_M…
 7 ensembl_exon_id               Exon stable ID         hsapiens_ge… ENSEMBL_M…
 8 study_external_id             Study external refere… hsapiens_ge… ENSEMBL_M…
 9 go_id                         GO term accession      hsapiens_ge… ENSEMBL_M…
10 dbass3_id                     DataBase of Aberrant … hsapiens_ge… ENSEMBL_M…
11 dbass5_id                     DataBase of Aberrant … hsapiens_ge… ENSEMBL_M…
12 hgnc_id                       HGNC ID                hsapiens_ge… ENSEMBL_M…
13 protein_id                    INSDC protein ID       hsapiens_ge… ENSEMBL_M…
14 mim_morbid_description        MIM morbid description hsapiens_ge… ENSEMBL_M…
15 mim_morbid_accession          MIM morbid accession   hsapiens_ge… ENSEMBL_M…
16 mirbase_id                    miRBase ID             hsapiens_ge… ENSEMBL_M…
17 refseq_peptide                RefSeq peptide ID      hsapiens_ge… ENSEMBL_M…
18 refseq_peptide_predicted      RefSeq peptide predic… hsapiens_ge… ENSEMBL_M…
19 wikigene_id                   WikiGene ID            hsapiens_ge… ENSEMBL_M…
20 mobidblite                    MobiDBLite             hsapiens_ge… ENSEMBL_M…

Now, all attribute names having id as part of their name are being returned.

Another example is topic = "homolog".

# show all elements of the data.frame
options(tibble.print_max = Inf)
# search for attribute topic "homolog"
head(biomartr::organismAttributes("Homo sapiens", topic = "homolog"), 20)
  <chr>                                         <chr>           <chr>   <chr> 
 1 mspretus_homolog_ensembl_gene                 Algerian mouse… hsapie… ENSEM…
 2 mspretus_homolog_associated_gene_name         Algerian mouse… hsapie… ENSEM…
 3 mspretus_homolog_ensembl_peptide              Algerian mouse… hsapie… ENSEM…
 4 mspretus_homolog_chromosome                   Algerian mouse… hsapie… ENSEM…
 5 mspretus_homolog_chrom_start                  Algerian mouse… hsapie… ENSEM…
 6 mspretus_homolog_chrom_end                    Algerian mouse… hsapie… ENSEM…
 7 mspretus_homolog_canonical_transcript_protein Query protein … hsapie… ENSEM…
 8 mspretus_homolog_subtype                      Last common an… hsapie… ENSEM…
 9 mspretus_homolog_orthology_type               Algerian mouse… hsapie… ENSEM…
10 mspretus_homolog_perc_id                      %id. target Al… hsapie… ENSEM…
11 mspretus_homolog_perc_id_r1                   %id. query gen… hsapie… ENSEM…
12 mspretus_homolog_goc_score                    Algerian mouse… hsapie… ENSEM…
13 mspretus_homolog_wga_coverage                 Algerian mouse… hsapie… ENSEM…
14 mspretus_homolog_dn                           dN with Algeri… hsapie… ENSEM…
15 mspretus_homolog_ds                           dS with Algeri… hsapie… ENSEM…
16 mspretus_homolog_orthology_confidence         Algerian mouse… hsapie… ENSEM…
17 vpacos_homolog_ensembl_gene                   Alpaca gene st… hsapie… ENSEM…
18 vpacos_homolog_associated_gene_name           Alpaca gene na… hsapie… ENSEM…
19 vpacos_homolog_ensembl_peptide                Alpaca protein… hsapie… ENSEM…
20 vpacos_homolog_chromosome                     Alpaca chromos… hsapie… ENSEM…

Or topic = "dn" and topic = "ds" for dn and ds value retrieval.

# show all elements of the data.frame
options(tibble.print_max = Inf)
# search for attribute topic "dn"
head(biomartr::organismAttributes("Homo sapiens", topic = "dn"))
  name                  description            dataset               mart      
  <chr>                 <chr>                  <chr>                 <chr>     
1 cdna_coding_start     cDNA coding start      hsapiens_gene_ensembl ENSEMBL_M…
2 cdna_coding_end       cDNA coding end        hsapiens_gene_ensembl ENSEMBL_M…
3 mspretus_homolog_dn   dN with Algerian mouse hsapiens_gene_ensembl ENSEMBL_M…
4 vpacos_homolog_dn     dN with Alpaca         hsapiens_gene_ensembl ENSEMBL_M…
5 pformosa_homolog_dn   dN with Amazon molly   hsapiens_gene_ensembl ENSEMBL_M…
6 cpalliatus_homolog_dn dN with Angola colobus hsapiens_gene_ensembl ENSEMBL_M…
# show all elements of the data.frame
options(tibble.print_max = Inf)
# search for attribute topic "ds"
head(biomartr::organismAttributes("Homo sapiens", topic = "ds"))
  name                description            dataset               mart        
  <chr>               <chr>                  <chr>                 <chr>       
1 ccds                CCDS ID                hsapiens_gene_ensembl ENSEMBL_MAR…
2 cds_length          CDS Length             hsapiens_gene_ensembl ENSEMBL_MAR…
3 cds_start           CDS start              hsapiens_gene_ensembl ENSEMBL_MAR…
4 cds_end             CDS end                hsapiens_gene_ensembl ENSEMBL_MAR…
5 mspretus_homolog_ds dS with Algerian mouse hsapiens_gene_ensembl ENSEMBL_MAR…
6 vpacos_homolog_ds   dS with Alpaca         hsapiens_gene_ensembl ENSEMBL_MAR…

Analogous to the organismAttributes() function, the organismFilters() function returns all filters that are available for a query organism of interest.

# show all elements of the data.frame
options(tibble.print_max = Inf)
# return available filters for "Homo sapiens"
head(biomartr::organismFilters("Homo sapiens"), 20)
   name                                description          dataset    mart    
   <chr>                               <chr>                <chr>      <chr>   
 1 chromosome_name                     Chromosome/scaffold… hsapiens_… ENSEMBL…
 2 start                               Start                hsapiens_… ENSEMBL…
 3 end                                 End                  hsapiens_… ENSEMBL…
 4 band_start                          Band Start           hsapiens_… ENSEMBL…
 5 band_end                            Band End             hsapiens_… ENSEMBL…
 6 marker_start                        Marker Start         hsapiens_… ENSEMBL…
 7 marker_end                          Marker End           hsapiens_… ENSEMBL…
 8 encode_region                       Encode region        hsapiens_… ENSEMBL…
 9 strand                              Strand               hsapiens_… ENSEMBL…
10 chromosomal_region                  e.g. 1:100:10000:-1… hsapiens_… ENSEMBL…
11 with_ccds                           With CCDS ID(s)      hsapiens_… ENSEMBL…
12 with_chembl                         With ChEMBL ID(s)    hsapiens_… ENSEMBL…
13 with_clone_based_ensembl_gene       With Clone-based (E… hsapiens_… ENSEMBL…
14 with_clone_based_ensembl_transcript With Clone-based (E… hsapiens_… ENSEMBL…
15 with_dbass3                         With DataBase of Ab… hsapiens_… ENSEMBL…
16 with_dbass5                         With DataBase of Ab… hsapiens_… ENSEMBL…
17 with_ens_hs_transcript              With Ensembl Human … hsapiens_… ENSEMBL…
18 with_ens_hs_translation             With Ensembl Human … hsapiens_… ENSEMBL…
19 with_entrezgene_trans_name          With EntrezGene tra… hsapiens_… ENSEMBL…
20 with_embl                           With European Nucle… hsapiens_… ENSEMBL…

The organismFilters() function also allows users to search for filters that correspond to a specific topic or category.

# show all elements of the data.frame
options(tibble.print_max = Inf)
# search for filter topic "id"
head(biomartr::organismFilters("Homo sapiens", topic = "id"), 20)
   name                          description               dataset     mart    
   <chr>                         <chr>                     <chr>       <chr>   
 1 with_protein_id               With INSDC protein ID ID… hsapiens_g… ENSEMBL…
 2 with_mim_morbid               With MIM morbid ID(s)     hsapiens_g… ENSEMBL…
 3 with_refseq_peptide           With RefSeq peptide ID(s) hsapiens_g… ENSEMBL…
 4 with_refseq_peptide_predicted With RefSeq peptide pred… hsapiens_g… ENSEMBL…
 5 ensembl_gene_id               Gene stable ID(s) [e.g. … hsapiens_g… ENSEMBL…
 6 ensembl_gene_id_version       Gene stable ID(s) with v… hsapiens_g… ENSEMBL…
 7 ensembl_transcript_id         Transcript stable ID(s) … hsapiens_g… ENSEMBL…
 8 ensembl_transcript_id_version Transcript stable ID(s) … hsapiens_g… ENSEMBL…
 9 ensembl_peptide_id            Protein stable ID(s) [e.… hsapiens_g… ENSEMBL…
10 ensembl_peptide_id_version    Protein stable ID(s) wit… hsapiens_g… ENSEMBL…
11 ensembl_exon_id               Exon ID(s) [e.g. ENSE000… hsapiens_g… ENSEMBL…
12 dbass3_id                     DataBase of Aberrant 3' … hsapiens_g… ENSEMBL…
13 dbass5_id                     DataBase of Aberrant 5' … hsapiens_g… ENSEMBL…
14 hgnc_id                       HGNC ID(s) [e.g. HGNC:10… hsapiens_g… ENSEMBL…
15 protein_id                    INSDC protein ID(s) [e.g… hsapiens_g… ENSEMBL…
16 mim_morbid_accession          MIM morbid accession(s) … hsapiens_g… ENSEMBL…
17 mirbase_id                    miRBase ID(s) [e.g. hsa-… hsapiens_g… ENSEMBL…
18 refseq_peptide                RefSeq peptide ID(s) [e.… hsapiens_g… ENSEMBL…
19 refseq_peptide_predicted      RefSeq peptide predicted… hsapiens_g… ENSEMBL…
20 wikigene_id                   WikiGene ID(s) [e.g. 1]   hsapiens_g… ENSEMBL…

Construct BioMart queries with biomartr

The short introduction to the functionality of organismBM(), organismAttributes(), and organismFilters() will allow users to perform BioMart queries in a very intuitive organism centric way. The main function to perform BioMart queries is biomart().

For the following examples we will assume that we are interested in the annotation of specific genes from the Homo sapiens proteome. We want to map the corresponding refseq gene id to a set of other gene ids used in other databases. For this purpose, first we need consult the organismAttributes() function.

# show all elements of the data.frame
options(tibble.print_max = Inf)

head(biomartr::organismAttributes("Homo sapiens", topic = "id"))
 name                          description                  dataset    mart   
  <chr>                         <chr>                        <chr>      <chr>  
1 ensembl_gene_id               Gene stable ID               hsapiens_… ENSEMB…
2 ensembl_gene_id_version       Gene stable ID version       hsapiens_… ENSEMB…
3 ensembl_transcript_id         Transcript stable ID         hsapiens_… ENSEMB…
4 ensembl_transcript_id_version Transcript stable ID version hsapiens_… ENSEMB…
5 ensembl_peptide_id            Protein stable ID            hsapiens_… ENSEMB…
6 ensembl_peptide_id_version    Protein stable ID version    hsapiens_… ENSEMB…
# show all elements of the data.frame
options(tibble.print_max = Inf)
# retrieve the proteome of Homo sapiens from refseq
file_path <- biomartr::getProteome( db       = "refseq",
                                    organism = "Homo sapiens",
                                    path     = file.path("_ncbi_downloads","proteomes") )

Hsapiens_proteome <- biomartr::read_proteome(file_path, format = "fasta")

# remove splice variants from id
gene_set <- unlist(sapply(strsplit(Hsapiens_proteome@ranges@NAMES[1:5], ".",fixed = TRUE), function(x) x[1]))

result_BM <- biomartr::biomart( genes      = gene_set, # genes were retrieved using biomartr::getGenome()
                                mart       = "ENSEMBL_MART_ENSEMBL", # marts were selected with biomartr::getMarts()
                                dataset    = "hsapiens_gene_ensembl", # datasets were selected with biomartr::getDatasets()
                                attributes = c("ensembl_gene_id","ensembl_peptide_id"), # attributes were selected with biomartr::getAttributes()
                                filters    = "refseq_peptide") # specify what ID type was stored in the fasta file retrieved with biomartr::getGenome()

result_BM 
  refseq_peptide ensembl_gene_id ensembl_peptide_id
1      NP_000005 ENSG00000175899    ENSP00000323929
2      NP_000006 ENSG00000156006    ENSP00000286479
3      NP_000007 ENSG00000117054    ENSP00000359878
4      NP_000008 ENSG00000122971    ENSP00000242592
5      NP_000009 ENSG00000072778    ENSP00000349297

The biomart() function takes as arguments a set of genes (gene ids specified in the filter argument), the corresponding mart and dataset, as well as the attributes which shall be returned.

Gene Ontology

The biomartr package also enables a fast and intuitive retrieval of GO terms and additional information via the getGO() function. Several databases can be selected to retrieve GO annotation information for a set of query genes. So far, the getGO() function allows GO information retrieval from the Ensembl Biomart database.

In this example we will retrieve GO information for a set of Homo sapiens genes stored as hgnc_symbol.

GO Annotation Retrieval via BioMart

The getGO() function takes several arguments as input to retrieve GO information from BioMart. First, the scientific name of the organism of interest needs to be specified. Furthermore, a set of gene ids as well as their corresponding filter notation (GUCA2A gene ids have filter notation hgnc_symbol; see organismFilters() for details) need to be specified. The database argument then defines the database from which GO information shall be retrieved.

# show all elements of the data.frame
options(tibble.print_max = Inf)
# search for GO terms of an example Homo sapiens gene
GO_tbl <- biomartr::getGO(organism = "Homo sapiens", 
                          genes    = "GUCA2A",
                          filters  = "hgnc_symbol")

GO_tbl

Hence, for each gene id the resulting table stores all annotated GO terms found in Ensembl Biomart.